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mmlv buffer

Contains 1 ml M-MLV Reverse Transcriptase Buffer and 500 µl DTT (0.1 M). ������N�\�A%։G����Ec����� 2c"d���3!�~Ĕ`���&���%�&/��.

Elle doit être impérativement vérifiée pour accéder à toutes les fonctionnalités de votre compte Promega.com. Isolated from a recombinant source Supplied with 10X Reaction Buffer Tested for the absence of DNases and RNases 0000002867 00000 n 0000055894 00000 n Outside of the United States, this product is intended for research use only unless otherwise stated. Veuillez vérifier les paramètres de votre réseau et réessayer. 1X M-MuLV Reverse Transcriptase Reaction Buffer 50 mM Tris-HCl 75 mM KCl 3 mM MgCl 2 10 mM DTT (pH 8.3 @ 25°C) Storage Temperature-20°C Storage Conditions. Pour protéger votre vie privée, votre compte sera verrouillé après 6 tentatives infructueuses. Simple spin or vacuum-based method to extract total RNA from tissues, cultured cells and white blood cells.

M-MLV Reverse Transcriptase Buffer is supplied as 5X Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl 2] with 500 µl 0.1 M DTT.

J. Hum. endstream endobj startxref Veuillez réessayer ou contacter le Service Client. Veuilez complèter votre compte à partir du mail que vous venez de recevoir, Vous ne pourrez pas avoir acès a votre compte si le mail n'est pas vérifié, Un compte existe déjà avec cette adresse email.

Erreur est survenue lors de la réinitialisation du mot de passe, Erreur de vérification du courrier électronique. Edit this message by logging in to your account, Note: Product prices and availability are subject to change without notice, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Chlorine and pH Test Kits and Test Strips, Applied Biosystems™ TaqMan® Assay and Arrays Search Tool, Applied Biosystems™ TaqMan® Custom Assay Design Tools, Applied Biosystems™ Custom qPCR Primers and TaqMan® Probes Tool. This buffer does contain actinomycin D, nucleotides, or dithiothreitol NOT (DTT). Amplifiable total RNA from FFPE tissues without organic solvents or overnight digestion. Fisher Scientific is always working to improve our content for you. DNA Seq Library Prep kits using Transposase, DNA-Seq Library Prep Kits without using Transposase, Library Indexing Primers and Adapter Primers, DNA isolation from Blood, Tissue and Cells, Biosafety Cabinets (BSC) and Balance Enclosures. 101 0 obj <>stream 0000006007 00000 n ��$�Gڦ�:��"j��#פ�����ܱN��HU�9MR��r��u���A���;w��g�:�ܔ �y`h2�J��P��yP�V^�7�u�[���3pH3�1/��h~HK�޹>-O���Q��M �S�\�,o(g_V�T�.��I�Q������p��ڂnl�L9��fl�`��I���Z���q�v�n�>h�7���0˱�i)g|��X�_1��u�Vu4��! 5 0 obj << /Linearized 1 /O 7 /H [ 959 201 ] /L 56559 /E 56002 /N 1 /T 56342 >> endobj xref 5 24 0000000016 00000 n Trademarks

0000028504 00000 n if excessively used ,the salt and Random primers in first reaction solution will restrain the activity of DNA polymerase .if purification needed ,it can follow the next :after reaction end of cDNA synthesis (step 6) , add RNase A in reaction system , 10 min in 37℃ ,use DP1501 recover cDNA .2. add next solution by order . Incubate at 42℃ for 60min(if use a random primer,first incubate for 10min in 25℃6.

Certificate of Analysis M-MLV Reverse Transcriptase: Part No. 28025-013.

0000028064 00000 n Un email de validation a été envoyé à l'adresse email associée à votre compte. Le mot de passe ne répond pas aux exigences.

No. M1701.

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Reaction requirements The M-MuLV reverse transcriptase reaction requires a primer. Genet. For Research Use Only. Transfer 10% volume of first reaction solution (2 μl ) to a proper PCR tube .note: the first reaction solution can be directly used as PCR template without purification ,the dosage is about 1-5μl. Store at -20°C. Veuillez réessayer ou contacter le Service Client. h�b``�f``�����`m��ˀ �L�@q� W����+������ExD�U|���2ۄ�[���*0�}dd��{��ȇ)�`�2L�Ҍ@�n� �R��#� 3� Identification des lignées cellulaires (STR), Méthodes automatisées pour la purification d'Acides nucléiques, Calculatrices + Outils d'analyse de données, POLITIQUE DE CONFIDENTIALITE ET DEMANDES DE RENSEIGNEMENTS, Conditions générales de vente - Promega France, Enzyme supplied at a concentration of 200u/μl. Recherche et développement de molécules cibles, Information sur l'utilisation des produits. h�bbd``b`:$�A� �S$XeA�a �v� The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases. 0 H��Wms�6���_�/7�J�;��I�\. Description. Not for use in diagnostic procedures. TRT-Lot No. © 2020 Promega Corporation, an affiliate of Promega France.

0000028313 00000 n 0000002828 00000 n

RNA-Dependent DNA Polymerase for cDNA Synthesis. 10,000u 50,000u 2 × 1ml. Le mot de passe a été utilisé trop récemment. O����guV���������U﫫�����S5�n���ZT��=෸��ժZ�Ϯ�j]}���=_'��ӟ'`&(:���۟����{N��n~wt����O��՚�T��������3��L,j�,��Ԣ8�z�iհ������?�ǘ?��:�OZq��g�� ���}G��"���)~m��o�n8�G�m�ǔ���]�o��z����.ngwO7�H�����C�8�����A��w��!M�)�l�-?w��&�Jn�c�/89�O�r�&���-ˠL��S��r|X�[ϸOқ�5�dY�'i�w�҃��9%�HY�jIJl�=̳���#���m]J��^��ʣ�c�ҷ����=��������֘�+@ƑR3��e�#�.�x�`�m͕�:���R�,��bغLW�R0��zM����20Es�X����t_�p�T2�� ����)l��e��g��@b둋l=�=��Dv�g�䞇s��݇(�~F$�ۧ/>���f���_� ��)���5�{~��ib~?J�_ɹ���\�7�IF�?AO\9�����я�[[3�m?�Bd��J>kn��0�k�r��Fj��m3.

0000001479 00000 n Use for double-stranded cDNA synthesis from RNA and subsequent ligation into a suitable vector. For use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), Cat.

H��V�n7}߯�#Yti�}�\-� �i��(YB�Z�#�1����p/ڵ����z������wկ��:Y\��#�~��N�����F�����n�n E��)+�M�N^�:�Z���R�F��] Catalog number selected: M1701. M-MLV RT Buffer Cat.

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The enzyme is an RNA-dependent DNA polymerase that uses single-stranded RNA or DNA as a template in the presence of a primer to synthesize a complementary DNA strand. ,� �D|�]�P 1{+����t��)Ȏ�@b�9#�! Vous venez de créer votre compte Promega.

使用制限 For Laboratory Use.

50 mM Tris-HCl 150 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% NP-40 pH 7.6 @ 25°C . Inactivate at 70℃ for 10min.PCR Reaction1. Your password reset link has expired. Un problème s'est produit lors de la vérification de votre adresse e-mail. Choose language: Download SDS PDF (411 KB) – 日本語 (日本) 分析証明書. 联系Promega及经销商 . The MMLV Reverse Transcriptase(short name MMLV) i is iolated from E.coli carrying rat leukemia virus pol gene consists of a single peptide, molecule weight 71kd.It possesses RNA and DNA depended polymerase activity and normal RNase H activity. Compared within AMV (avian myeloblastosis virus) reverse transcriptase, … �Hi��ɍt�C�xcA^�?

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PCR & Real-Time PCR, Reverse Transcription.

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Mix thoroughly and add 50μl mineral oil to the surface of liquid.4. 0000000959 00000 n

Get in touch with a nearby distributor or sales representative, Catalog number selected: Purifies RNA from cells using a low elution volume and the Maxwell® RSC Instrument. The enzyme is expressed in E. coliHB101 pB6B 15.23.

0000001311 00000 n

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%PDF-1.3 %���� Please request another reset link. Après cela, vous devrez contacter le Service Client pour le déverrouiller. Veuillez réessayer ou contacter le Service Client. 0000002239 00000 n It possesses RNA and DNA depended polymerase activity and RNase H activity. 5μl 10X PCR Buffer 1μl 10mM dNTP mix 1μl 10μM Primer #1 (customer supplied) 1μl 10μM Primer #2 (p customer supplied) xμl H20 (total reaction volume:49μl) 1μl Taq DNA polymerase3. Please sign in to view account pricing and product availability. 0000002655 00000 n Un problème est survenu lors de la réinitialisation de votre mot de passe. 0000006298 00000 n M-MLV Reverse Transcriptase Buffer is supplied as a [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl, It is supplied with M-MLV, SuperScript, SuperScript II, and SuperScript III reverse transcriptases.

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0000005404 00000 n

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Helpful for preparing long cDNAs and libraries with a high percentage of full-length cDNA.

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single- stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. Enzyme Buffer Pack. Conditions générales de vente - Promega France 51 0 obj <> endobj Transcribes cDNA from RNA in as little as 15 minutes. You are viewing: M1705 Change Configuration.

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